twentyfourteen domain was triggered too early. This is usually an indicator for some code in the plugin or theme running too early. Translations should be loaded at the init action or later. Please see Debugging in WordPress for more information. (This message was added in version 6.7.0.) in /var/www/vhosts/gcsar.gov.sy/httpdocs/en/wp-includes/functions.php on line 6131Nour Ghazi Al-Asaad <\/strong> Abstract<\/strong><\/p>\n In the current study, analysis for some field crops, feed and food products found in the local market was conducted for GMOs detection using molecular biology techniques based on PCR, Multiplex PCR, and Nested PCR. DNA was extracted from samples including: corn, soybean, sunflower, barley, tomato, cucumber, pop corn, and rice followed by tests to ensure purity of DNA samples using specific primers, such as detection of Lactin and zein genes which are specific to soybean and maize respectively. Nour Ghazi Al-Asaad General Commission for Scientific Agricultural Research- Biotechnology Department Faculty of Agriculture, Damascus University, 2010 Abstract In the current study, analysis for some field crops, feed and food products found in the local market was conducted for GMOs detection using molecular biology techniques based on PCR, Multiplex PCR, and Nested PCR. DNA was … Continue reading Detection of Genetically Modified Organisms in Some Products from the Local Syrian Markets Using Molecular Biology Techniques<\/span>
\nGeneral Commission for Scientific Agricultural Research-<\/strong>
\nBiotechnology Department<\/strong>
\nFaculty of Agriculture, Damascus University, <\/strong>
\n2010<\/strong><\/p>\n
\nGMO detection in the studied samples was conducted using specific primers for the most commonly used foreign DNA in genetic transformation such as 35 S promoter from cauliflower mosaic virus, Nopaline synthase terminator (T-Nos), the gene which confers tolerance to glyphosate-herbicide, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), CRYIA(b) gene which confer resistance to insects, NPTII\u00a0gene which confer resistance to kanamycine, BAR\u00a0gene which gives tolerance to glyphosate-herbicide, and the selectable marker gus gene, using the following plasmids as positive control: PGIIMH35S-g2ps1, pBI221, TOP10 PGII35S CRYA(b) ,TOP10 PGII35S CRYA(c).
\nTests revealed existence of GMOs in two imported products such as corn, and soybean due to the presence of 35S promoter and NOS\u00a0terminator, and EPSPS\u00a0gene in soybean, while tests showed negative results in the local crops tested. Bioinformatics tools and nested PCR were used to confirm our findings.<\/p>\n","protected":false},"excerpt":{"rendered":"