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Characterization and vegetative propagation of some prunus cerasia Blanche types prevailing on the syrian coast | General Commission for Scientific Agricultural Research
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Characterization and vegetative propagation of some prunus cerasia Blanche types prevailing on the syrian coast

Mohammad Qais Nizam
Department of Horticulture, General Commission for Scientific Agricultural Research (GCSAR)
Tishreen University.
2018

Abstract

     This research was conducted during 2016-2018 on (42) wild types of Prunus cerasia Blanche prevailing in 12 sites of Lattakia and Tartous provinces. The results showed obvious morphological differences between the studied types according to morphological affinity tree at 33.65%. The studied types were distributed to four groups.
Investigated pollen grains were similar: spherical, medium-sized and tricolporate, while they varied in the average lengths of polar and equator axes. LRsh1 and LRsh2 types in Ras al-Shamra site were more significant than others. The investigated types were also variant in the dimensions of anthers and number of pollen grains within the anther.
The results also showed variation in the chemical content of studied traits of wild plum fruits, so the fruit content of soluble solids had the highest value 23% for the type (LK3) in Kassab site and the highest total acidity was 3.06% for the type (TM1) in Matn al-Sahel site. As for fruit content of sugars, the two types (LD1 and LD2) in Al-Daliya site had a good content of sugars (8.83 and 8.9%) respectively, and they were significantly higher than other studied types. In addition, significant variation between plum types in terms of fruit content of dry matter was found, ranging between (12.07-25.18%).
Ten Simple Sequence Repeat primers (SSR) were used to determine genetic variation of considered types. Eight primers showed clear genetic variation and generated 28 alleles, of which 16 were polymorphic at (57.14%) of polymorphism.                                        According to affinity tree, the genetic variation of considered types ranged between 0 and 30%.
The stem cuttings (semi-wood cuttings) of considered types were treated with different concentrations of IBA (1000 – 2000 – 4000) ppm and the control. The findings stated that the treatment (2000) ppm was the most significant: giving the highest number of roots and root length. The trreatments (2000 – 4000) ppm gave the best findings for rooting percentage vs the treatment 1000 ppm. The types (LK1, LK2) from Kassab site gave the best findings in terms of rooting percentage and root length, while there were no significant differences between all investigated types in terms of root number on stems.
The initial compatibility of a collection of economically important almonds cultivars (plum, peach and apricot) and P. cerasia rootstock was determined. The findings showed that the plum cultivar was more significant than apricot and peach cultivars in terms of grafting survival percentage, average length and thickness of shoots for both tongue grafting and T-budding methods. In addition, the apricot cultivar was more significant than peach cultivar in terms of grafting survival percentage and shoots length. The peach was more significant than studied apricot cultivar in terms of average shoots thickness. LK1 and LK2 in Kassab showed the best grafting results, where the grafting survival percentage, average shoot length and thickness were the highest for grafting with plum cultivar (86.66%, 48.16 cm, 3.9 mm) and (100%, 57 cm, 6.16 mm), respectively for both grafting methods.

      Key words: Wild plum, morphological characterization, pollen grains, SSR markers, semi-wood cuttings, grafting.

 

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